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SCI 115 Week 6 Lab Quiz

SCI 115 Week 6 Lab Quiz


1. Most scientists who use DNA microarrays obtain them


from   companies that mass produce them


from   the government


by   making them in their labs


by   exploiting ambitious graduate students


1. “In this study, cancerous and normal skin tissue samples were excised with a scalpel. Each skin sample was then placed into a sample tube along with solvent, shaken on a vortex and centrifuged. When the sample tubes came out of the centrifuge, there was a top layer and a bottom layer. For our purposes, we went on to use ______________ for further processing.”


only   the top layer


only   the bottom layer


a   mixture of top and bottom layers


none   of the options listed


1. The purpose of the vortex instrument is to


vigorously   shake the tissue sample in the solvent so that it dissolves.


spin   the tissue/solvent mixture around to separate the solids


cause   the mRNA to bind to the pellets


dispose   of biohazardous waste safely


1. “Once the RNA is isolated from the DNA, proteins and other materials, the solvent/RNA mixture is run through a column of all beads. Each bead has several short strands of polyT RNA sticking out of it. In this step:”


“mRNA   sticks to the beads, while rRNA and tRNA run right through the column”


“tRNA   sticks to the beads, while mRNA and rRNA run right through the column”


“rRNA   and tRNA stick to the beads, while mRNA runs right through the column”


none   of the options listed


1. “Once the mRNA is isolated, you make a DNA copy of it using by adding polyT primers, labeled DNA nucleotides, and an enzyme known as:”


reverse   transcriptase


tyrosine   hydroxylase


alcohol   dehydrogenase


ATP   kinase


1. A DNA copy of a mRNA transcript is known as


complementary   DNA (abbreviated cDNA)


copied   DNA (abbreviated cDNA)


mitochondrial   DNA (abbreviated mtDNA)


recombinant   DNA (abbreviated rDNA)


1. Each spot on the DNA microarray in embedded with


copies   of DNA from one particular gene


copies   of DNA from several different genes with similar functions


copies   of DNA from several different genes with different functions


copies   of mRNA from one or more genes


1. “When we say that the cDNA derived from our sample has hybridized to a particular spot on the array, we mean that: ”


The   cDNA from the sample has stuck to the DNA on the microarray at that point.


The   cDNA from the sample is jumbled and cannot be trusted.


The   cDNA has been used to make an mRNA copy


You   need to buy microarrays from different manufacturers and triangulate the   results


1. “In this particular experiment, we used red-labeled DNA to process the sample from _____________ tissue and the green-labeled DNA to process the sample from ___________. ”


cancerous;   normal


normal;   cancerous


normal;   normal


cancerous;   cancerous


1. “After scanning the green labeled areas and the red labeled areas, when we combine the two images, the spots that show up as yellow correspond to ”


genes   expressed by both normal or cancerous skin cells.


genes   that were not expressed at all in either normal or cancerous skin cells


“genes   expressed by cancerous, but not normal skin cells”


“genes   expressed by normal, but not cancer skin cells. ”


1. “In interpreting the results of this study, spots on the microarray that are red correspond to:”


genes   that are turned up by cancer


genes   that are turned down by cancer


genes   that are unaffected by cancer


genes   that aren t expressed in normal or cancerous cells


1. “In interpreting the results of this study, spots on the the microarray that are green correspond to genes that are ”


genes   that are turned down by cancer


genes   that are turned up by cancer


genes   that are unaffected by cancer


genes   that aren t expressed in normal or cancerous cells


1. “When the DNA microarray study tells us that a large number of genes have been turned up (or turned down) by a disorder, the most likely explanation is that ”


the   turned up genes are likely controlled by a gene that has gone bad


all   of these genes are genes that have gone bad themselves


even   just one mutation in any of these affected genes would have been sufficient   to cause the disorder


the   cytoplasm has too many free radicals in it


1. A gene shown by the microarray to be expressed is :


“probably   making protein, but a protein expression analysis would be needed to know for   sure”


definitely   making protein


definitely   not making protein


probably   making protein but there’s no way to know for sure


1. “In the application of this technique to skin cancer, a gene that has gone bad ”


“may   or may not be identified at all, but the overall pattern of results can give   important clues”


can   almost always be identified via DNA microarray

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