OBJECTIVESThe rationale of this experiment is to determine the relative rates of cellular respiration among germinated and non-germinated peas, cold and room temperature peas, and insects. This can be determined by measuring the amount of oxygen consumed and the amount of carbon dioxide that is released during a given amount of time.In each specimen-we want to observe the changes in the O2 (ppm/sec) from the beginning to the end of the experimental set up-if we observe decline in the total amount of O2 registered in the lab quest (so more O2 at the beginning of the experiment and less O2 at the end of the experiment) the organism in the bio chamber must be respiring-because it is utilizing the O2. Similarly, we also want to observe the changes in the CO2 (ppm/sec) from the beginning to the end of the experimental set up -if there is increase in CO2 registered in the lab quest (so less CO2 at the beginning of the experiment and more CO2 at the end of the experiment) – the organism in the bio chamber must be respiring-because it is exhaling the CO2. Additionally, we want to investigate how manipulating independent variable changes the dependent variable. Hence, identify independent variable, dependent variable, constant and replicates for each experiment (Review Chapter 1 in Lab Manual).
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